Circular dichroism (CD) spectroscopy measures the absorption difference between left and right circularly polarized light. A molecule containing a chiral chromophore or if locate in a chiral environment can exhibit a CD signal.

CD can be used for

• Characterization of secondary and tertiary protein structure.
• Topology of nucleic acids structures (A-helix, B-helix, Z-helix, G-quadruplexes structure), and their complexes with small molecules.
• Synthetic molecules in solution.
• Estimation of the contents in secondary structure (alpha-helix, beta-sheet) in sample.
• Control of the folding  of molecule.
• Study of conformational changes (as a function of time, solvent,…).
• Analysis of the stability of samples in their physicochemical environment.
• Analysis of ligands or lipids fixation on target molecule.

Equipements

• Jasco J-815

• Wavelength range : 183 to ~ 900 nm

• temperature range : 4 to 90 °C

Access

• The circular dichroism J-815 instrument can be operated only if the user has followed a training course dispensed by the technical manager of the CD platform. Contact the technical manager to have the training.
• Prices: please contact the technical manager for information.
  
  

Technical manager

Laëtitia Minder This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Scientific expert

Frédéric Rosu This e-mail address is being protected from spambots. You need JavaScript enabled to view it