Services and expertise

Protein expression assay

This test evaluates the level of expression and solubility of candidate proteins from different bacterial strains (8 strains of E. coli in total). A scale-up can be performed to evaluate the level of expression in different volumes. Plasmid constructs for expression assays may be provided either by the customer or generated by the facility.

Protein production and purification

This service offers the production and the purification of recombinant protein from a gene of interest. To allow easier purification, the gene of interest is cloned into a tagged vector. We carry out the expression of recombinant proteins in E. coli. Plasmid constructs containing sequence of interest may be provided either by the customer or by the facility.

Equipments

Centrifuges

• AVANTI J26XP (Beckman coulter) equipped with rotors JLA 8.1000, JA25.50.
• 5804R (Eppendorf) equipped with: Swing-bucker rotor for plates A-2-DWP, Standard rotor for 1,5/2ml tubes FA-45-30-11, Rotor F-34-6-38 (Adaptator for 15ml, 15-18ml or 50ml tubes).
• 5418 (Eppendorf) equipped with Rotor for 1,5/2ml tubes FA-45-18-11

Ultracentrifuges:

• OPTIMA-L80XP (Beckman coulter) equipped with rotors SW40Ti, 50.2 Ti.
• OPTIMA MAX (Beckman coulter) equipped with rotors: TLA 120, MLS 80, MLA 80.

Bacterial refrigerated incubator: MaxQ 6000 (Thermofisher)

Bacterial incubator: StabiliTherm (Thermofisher)

Technical contact

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Scientific expertise

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